Journal: Open Medicine

Article Title: Phospholipid transfer protein ameliorates sepsis-induced cardiac dysfunction through NLRP3 inflammasome inhibition

doi: 10.1515/med-2024-0915

Figure Lengend Snippet: Effect of rh PLTP on LPS-induced pyroptosis in mouse cardiomyocytes. (a) Cell viability was detected by the CCK-8 assay. n = 4, ** = P < 0.01, *** = P < 0.001. (b) LDH content in the cell culture supernatant was detected by the LDH release assay. n = 4, *** = P < 0.001. (c) Morphological changes of the M6200 cell membrane were observed using SEM. (d)–(j) Expression of PLTP, NLRP3, ASC, caspase-1, IL-1β, and GSDMD was detected by WB, and the relative expression was calculated from the gray-scan value and analyzed using GraphPad Prism. n = 3, *** = P < 0.001, # = P < 0.05, ## = P < 0.01.

Article Snippet: The membrane was blocked with 5% skim milk for 2 h and incubated with primary antibodies against PLTP (1:1,000, PK72825; Abmart), ASC (1:1,000, TP73812; Abmart), NLRP3 (1:1,000, T55651; Abmart), caspase-1 (1:1,000, TP70406; Abmart), IL-1β (1:1,000, PA1586; Abmart), GSDMD (1:1,000, TA4012; Abmart), and GAPDH (1:10,000, ab181602; Abcam) overnight at 4°C.

Techniques: CCK-8 Assay, Cell Culture, Lactate Dehydrogenase Assay, Membrane, Expressing

Journal: Open Medicine

Article Title: Phospholipid transfer protein ameliorates sepsis-induced cardiac dysfunction through NLRP3 inflammasome inhibition

doi: 10.1515/med-2024-0915

Figure Lengend Snippet: Effects of PLTP on the activation of the NLRP3 inflammasome/GS. DMD pathway in the heart tissue of mice with sepsis. (a)–(c) IHC was performed to evaluate the distribution of PLTP and NLRP3 in the heart tissue of mice, and the positive staining areas of PLTP and NLRP3 were analyzed. n = 5, *** = P < 0.001, ## = P < 0.01, ### = P < 0.001. (d)–(j) Expression of PLTP, NLRP3, ASC, caspase-1, IL-1β, and GSDMD was detected by WB, and the relative expression was calculated from the gray-scan value and analyzed using GraphPad Prism. The graph presents the densitometry results of three independent experiments (mean ± SD). n = 5, *** = P < 0.001, # = P . < 0.05, ## = P < 0.01, ### = P < 0.001.

Article Snippet: The membrane was blocked with 5% skim milk for 2 h and incubated with primary antibodies against PLTP (1:1,000, PK72825; Abmart), ASC (1:1,000, TP73812; Abmart), NLRP3 (1:1,000, T55651; Abmart), caspase-1 (1:1,000, TP70406; Abmart), IL-1β (1:1,000, PA1586; Abmart), GSDMD (1:1,000, TA4012; Abmart), and GAPDH (1:10,000, ab181602; Abcam) overnight at 4°C.

Techniques: Activation Assay, Staining, Expressing

Journal: Open Medicine

Article Title: Phospholipid transfer protein ameliorates sepsis-induced cardiac dysfunction through NLRP3 inflammasome inhibition

doi: 10.1515/med-2024-0915

Figure Lengend Snippet: Interaction between PLTP and NLRP3 proteins. (a) Interaction between PLTP and NLRP3 was predicted using the GeneMANIA database. (b) Predicted relationship between PLTP and NLRP3 was verified by Co-IP, n = 3. (c)–(i) Expression of PLTP, NLRP3, ASC, caspase-1, IL-1β, and GSDMD was detected by WB, and the relative expression was calculated from the gray-scan value and analyzed using GraphPad Prism. n = 3, ns = P > 0.05, * = P < 0.05, ** = P < 0.01, *** = P < 0.001.

Article Snippet: The membrane was blocked with 5% skim milk for 2 h and incubated with primary antibodies against PLTP (1:1,000, PK72825; Abmart), ASC (1:1,000, TP73812; Abmart), NLRP3 (1:1,000, T55651; Abmart), caspase-1 (1:1,000, TP70406; Abmart), IL-1β (1:1,000, PA1586; Abmart), GSDMD (1:1,000, TA4012; Abmart), and GAPDH (1:10,000, ab181602; Abcam) overnight at 4°C.

Techniques: Co-Immunoprecipitation Assay, Expressing